pgrna libraries Search Results


guide rna  (Addgene inc)
92
Addgene inc guide rna
Guide Rna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgrna+libraries/pmc05797955-433-8-19?v=Addgene+inc
Average 92 stars, based on 1 article reviews
guide rna - by Bioz Stars, 2026-07
92/100 stars
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pgrna library  (CustomArray Inc)
90
CustomArray Inc pgrna library
Generation of AsCpf1 <t>pgRNA</t> <t>library</t> targeting lncRNAs and assessment of plasmid pool quality. (A) Graphical depiction of AsCpf1 pgRNA library composition, pgRNA library generation in pRMCE-EpoFc-UP and DOWN version, and plasmid pool quality assessment by next-generation (NGS) sequencing. (B) Correlation plot of normalized pgRNA counts of the two different plasmid versions. (C) Bar plot of pgRNA sequence quality in the plasmid pools. Identification of pgRNAs with correct sequences, sequences containing variants or pgRNAs not present in the library version. (D) Bar plot of determined pgRNA sequence variants in plasmid libraries.
Pgrna Library, supplied by CustomArray Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgrna+libraries/pmc08261548-141-1-6?v=CustomArray+Inc
Average 90 stars, based on 1 article reviews
pgrna library - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
pgrna libraries  (Twist Bioscience)
86
Twist Bioscience pgrna libraries
Generation of AsCpf1 <t>pgRNA</t> <t>library</t> targeting lncRNAs and assessment of plasmid pool quality. (A) Graphical depiction of AsCpf1 pgRNA library composition, pgRNA library generation in pRMCE-EpoFc-UP and DOWN version, and plasmid pool quality assessment by next-generation (NGS) sequencing. (B) Correlation plot of normalized pgRNA counts of the two different plasmid versions. (C) Bar plot of pgRNA sequence quality in the plasmid pools. Identification of pgRNAs with correct sequences, sequences containing variants or pgRNAs not present in the library version. (D) Bar plot of determined pgRNA sequence variants in plasmid libraries.
Pgrna Libraries, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgrna+libraries/pm41881989-325-4-6?v=Twist+Bioscience
Average 86 stars, based on 1 article reviews
pgrna libraries - by Bioz Stars, 2026-07
86/100 stars
  Buy from Supplier

Image Search Results


Generation of AsCpf1 pgRNA library targeting lncRNAs and assessment of plasmid pool quality. (A) Graphical depiction of AsCpf1 pgRNA library composition, pgRNA library generation in pRMCE-EpoFc-UP and DOWN version, and plasmid pool quality assessment by next-generation (NGS) sequencing. (B) Correlation plot of normalized pgRNA counts of the two different plasmid versions. (C) Bar plot of pgRNA sequence quality in the plasmid pools. Identification of pgRNAs with correct sequences, sequences containing variants or pgRNAs not present in the library version. (D) Bar plot of determined pgRNA sequence variants in plasmid libraries.

Journal: Biotechnology Reports

Article Title: A pooled CRISPR/AsCpf1 screen using paired gRNAs to induce genomic deletions in Chinese hamster ovary cells

doi: 10.1016/j.btre.2021.e00649

Figure Lengend Snippet: Generation of AsCpf1 pgRNA library targeting lncRNAs and assessment of plasmid pool quality. (A) Graphical depiction of AsCpf1 pgRNA library composition, pgRNA library generation in pRMCE-EpoFc-UP and DOWN version, and plasmid pool quality assessment by next-generation (NGS) sequencing. (B) Correlation plot of normalized pgRNA counts of the two different plasmid versions. (C) Bar plot of pgRNA sequence quality in the plasmid pools. Identification of pgRNAs with correct sequences, sequences containing variants or pgRNAs not present in the library version. (D) Bar plot of determined pgRNA sequence variants in plasmid libraries.

Article Snippet: The pgRNA library was received from CustomArray Inc. as a 12,000 single-stranded oligo pool (5-fold representation per pgRNA), PCR amplified with a low number of cycles and further cloned into two versions of the delivery plasmid backbone pRMCE-EpoFc either up- or downstream of the model protein EpoFc yielding the two plasmid libraries UP-pgRNAs and DOWN-pgRNAs.

Techniques: Plasmid Preparation, Sequencing